E-Book 2nd Congress

  • Evaluation of probiotic effect of Lactobacillus Casei and Bacillus Coagulans on AGS and DU145 cancer cell line
  • Samaneh Ansarinia,1,* Parisa Behshood,2 Ali Sharifzadeh,3
    1. Azad univercity sh.k
    2. azad univercity sh.k
    3. azad univercity sh.k


  • Introduction: Probiotics are live microbial supplements with a wide range beneficial in human life. Nowadays, probiotics are known as a factor for prevention of infectious diseases and cancer.
  • Methods: In this work the inhibitory effect of the supernatant of an autochthonous isolate of Lactobacillus Casei and Bacillus Coagulans on the AGS and DU145 cell Lines was investigated. Different concentration of 100,200, and 300 µl/ml of the supernatant of the probiotic bacteria harvested at 24,48 and 72 hours of these growth in MRS media were applied in to the 96 well microplates each containing 8000 cells of AGS and DU145 cell lines after neutralization of the pH with 1N NaOH.
  • Results: The percentage of cancer cells inhibition observed from 1000 µg/ml (72h )and 300 µl (72h) supernatants
  • Conclusion: The inhibitory effect of the probiotic bacteria on human cancer cells seems to be concentrated dependent and not affected by neutralization.
  • Keywords: Probiotic,Gastric Cancer,Prostat Cancer