E-Book 2nd Congress

  • Efficient In Vitro Differentiation Of Hematopoietic Stem Cell Into Erythroid Progenitor Cells
  • Reyhaneh Abriyan,1,* Amir Atashi,2
    1. Student Research Committee, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran
    2. Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran


  • Introduction: Pure red cell aplasia is defined by a normocytic normochromic anemia with severe reticulocytopenia and marked reduction or absence of erythroid from the bone marrow. Abnormalities from pure red cell aplasia is limited only to the red cell lineage. The main cells involved in pure red cell aplasia are erythroid progenitor cells (EPCs). To perform cellular and molecular researches on this disease, EPCs in culture is required. In this study, EPCs were differentiated from hematopoietic stem cells using a highly efficient method.
  • Methods: For the generation of EPCs, CD34+ cells were isolated from mononuclear cells derived from human umbilical cord blood. The isolated cells were cultured for 14 days in erythroid differentiation medium consisting IMDM containing 20% fetal bovine serum, 10% heat-inactivated fetal bovine serum, 1X ITS, 50nM β-mercaptoethanol, supplemented by 25 ng/mL IL-3, 100 ng/mL SCF, and 4 IU/mL EPO.
  • Results: The cultured cells were showed morphological changes at the end of the culture period. The cells were grown as suspended colonies. Microscopic findings, GATA-1 and EPOR gene expression and specific cell surface marker analysis proved differentiation into erythroid lineage. Based on flow cytometry results, 86% of the differentiated cells were EPCs.
  • Conclusion: As the results are illustrative, the current differentiation method is an efficient method for production of EPCs in culture. These cells can be used in a wide range of researches of the red blood cell lineage specially to investigate molecular pathogenesis of red cell aplasia.
  • Keywords: Pure red cell aplasia, Hematopoietic Stem Cell, erythroid progenitor cells (EPCs), GATA-1, EPOR