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  • Phytochemical compounds and antifungal activity of aqueous and alcoholic extracts of artichoke herb (Cirsium vulgare) on Aspergillus flavus
  • Issa Gholampour Azizi,1 Ava Ghasemi Mianaei,2 Hossein Foadaddini,3,*
    1. Department of Mycology, Faculty of Veterinary Medicine, Islamic Azad University Babol Branch, Babol, Iran
    2. Department of veterinary, Babol Branch, Islamic Azad University, Babol, Iran
    3. Department of veterinary, Babol Branch, Islamic Azad University, Babol, Iran


  • Introduction: Given that one of the main problems today with pathogenic microorganisms is their increased resistance to antibiotics, many efforts are being made to obtain more information about the active ingredients in plants and their use in the treatment of diseases. This study the effect of aqueous and alcoholic extracts of Cirsium vulgare on Aspergillus flavus. In this study, the aqueous, ethanolic and methanolic extracts of Cirsium vulgare were studied on Aspergillus flavus with disc, wells, minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) concentrations.
  • Methods: It was collected from the stem and leaves of the artichoke plant in the plains of Babol city in the summer of 1401 and aqueous and alcoholic extracts were obtained by the Soxola method. Also, the essential oils of Cirsium vulgare were measured by gas chromatography. The average of growth inhibitory diameter for the aqueous, ethanol and methanol extracts in the disc method was 4.92, 12.50 and 11.25 mm, respectively. Also, in volumes of 60 and 70 microliters, a significant increase was observed in the average of growth inhibitory diameter between alcoholic and aqueous extracts.
  • Results: Based on the results of the present study, the average of growth inhibitory diameter in volumes of 90, 100 and 110 μl in the well method showed a significant difference between 3 different types of extracts; In a way, the aqueous extract showed a larger growth inhibitory diameter compared to the methanolic and ethanolic extracts. The mean amount of aqueous, ethanolic and methanolic extracts in MIC method was 1041.67 μg/ml and in MFC method was 25000.00, 29166.67 and 583333.33 μg/ml, respectively; There was no significant difference in MIC and MFC values.
  • Conclusion: According to the present study, artichoke plant extract can be used to eliminate Aspergillus mold due to the presence of chlorogenic acid, cynarin, lutein 7-O-rotinoside and cynarozoid.
  • Keywords: medicinal plants, Cirsium vulgare, Aspergillus flavus, antifungal activity