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  • Regulatory effects of the novel synthesized Indole-3-carbaldehyde on expression of cell cycle genes: A study on Cyclin D and P21 expression by acute promylocytic leukemia cell line (NB4)
  • ozra sadat esmaeili,1,* Mojgan Noroozi Karimabad,2 Gholamhossein Hassanshahi,3 Mohammad Reza Hajizadeh,4
    1. Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
    2. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
    3. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
    4. Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran


  • Introduction: A large variety of heterocycles are known to date and among these, indole and pyran rings are of particular interest. The present investigation was aimed to investigate the effects of novel Indole-3-carbaldehyde derivative (NI-3-CD) analogue on growth inhibition of cells and its regulatory effects on expression of cell cycle genes in acute promylocytic leukemia (APL) cell line.
  • Methods: NB4 cells were cultured in presence of RPMI1640 medium contained various concentrations of NI-3-CD and basal compound of indole (I3C) (15.12-1000 μg/mL) for 24, 48 and 72 hours. The inhibitory effects of compound on cellular proliferation were assessed by both trypan blue staining and MTT assay techniques. when was confirmed apoptosis the changes in expression of Cyclin D and P21 were determined by quantitative Real-Time PCR. Western blotting analysis was also applied for evaluating the expression of P21 at protein level. Differences were considered significant if p values less than 0.05.
  • Results: Our results showed a significant difference between various concentrations of NI-3-CD and I3C when cells were treated for 24, 48 and 72 h. Real Time- PCR analysis indicated that the expression of Cyclin D was down regulated while P21 upregulated in compare to untreated control cells and I3C treated cells(P < 0.01). In concert with RT-PCR, western blot analysis also showed that the P21 expression in NI-3-CD treated cells was significantly increased in compare to both untreated control cells and I3C treated cells.
  • Conclusion: According to these findings, the novel synthesized NI-3-CD analogue effects on the cell cycle arrest in APL cell line is possibly facilitated via modulating Cyclin D and P21 pathway mediators. NI-3-CD may introduce this compound as a promising therapeutic compound against APL.
  • Keywords: C19H15F3N2O; Gene; Cancer; Flow cytometry; Real-time PCR; Western blot.