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E-Book 2nd Congress

  • Investigating the pathway of resistance to Novobiocin in Campylobacter jejuni by using microarray analysis
  • Mahlagha Cheraghi,1,*
    1. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran


  • Introduction: C. jejuni is one of the main and effective Gram-negative bacteria in food poisoning and causing gastrointestinal infections in the intestine. This bacterium is a hidden microbe in food, so-called foodborne, which is able to cause infection by penetrating the small intestine, and for this reason, it has become one of the main sources of digestive and internal infections. The main challenge in dealing with C. jejuni infections is the resistance of strains of this species to various antibiotics. Until now, various antibiotics, including Novobiocin, have failed against the infection of this bacterial strain, and today, a combination of various antibiotics such as vanomycin, polymixin-B, and acitidone is used as an antibiotic cocktail in the treatment of this disease. Investigating gene expression profiles in antibiotic resistant strains of this disease can clarify the process of resistance and pathogenicity of resistant strains and play a role in designing new drugs, prescribing antibiotics and finally eradicating C. jejuni infections be effective.
  • Methods: The GSE18415 dataset has been used to investigate resistance to the Novobiocin antibiotic. After downloading the data and performing the initial preprocessing and normalization, the analyzes previously reviewed on ciprofloxacin resistance and for these data are also run. Only because the microarray data is normal, the limma library is used this time.
  • Results: For this dataset, the design of the experiment consists of two control groups and treated with a concentration of 256 micrograms of Novobiocin antibiotic, each group having 4 samples with both Cy3 and Cy5 colors so that color bias does not occur. Also, PCA analysis and principal component analysis also show a proper separation of the samples of the control and antibiotic treatment groups, which indicates a significant change in the gene expression profile of this bacterium in response to Novobiocin and as a result, the occurrence of antibiotic resistance.
  • Conclusion: With the established resistance to the Novobiocin antibiotic, as it is known, generally genes related to translation pathways that generally involve ribosomes and translation factors, genes related to movement, tag synthesis and bacterial motility and response proteins are down-regulate and proteins involved in protein degradation pathways and have protease activity, genes involved in substance transfer and genes of the oxidation and reduction chain family and cytochromes are up-regulated.
  • Keywords: Antibiotic resistance , Campylobacter jejuni , Microarray