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  • In vitro and ex vivo effects of Astragalus adscendens extract against hydatid cyst protoscoleces and its effect on induction of apoptosis
  • Amirreza Mousivand,1 Hossein Mahmoudvand,2,* Samin Ahmadi,3 Javad Ghasemian Yadegari,4 Maryam Beig Mohammadi,5 Mahtab Borjian Broujeni,6
    1. Student Research Committee, Faculty of paramedical sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
    2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
    3. Student Research Committee, Faculty of paramedical sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
    4. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
    5. Student Research Committee, Faculty of paramedical sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
    6. Student Research Committee, Faculty of paramedical sciences, Lorestan University of Medical Sciences, Khorramabad, Iran


  • Introduction: This study aims to evaluate in vitro and ex vivo antiparasitic activity of Astragalus adscendens root chloroformic extract against hydatid cyst protoscoleces and its effect on induction of apoptosis.
  • Methods: Various concentrations of the A. adscendens root chloroformic extract (56.25, 112.5, 225, and 450 mg/mL) were treated with hydatid cyst protoscoleces collected from the liver of infected sheep for 5-60 min in vitro and ex vivo. Eosin exclusion test was also utilized to measure the mortality of protoscoleces. Moreover, the extract effect was assessed on apoptosis induction in hydatid cyst protoscoleces by Caspase-3 activity measurement.
  • Results: The mortality rate of protoscoleces in in vitro was 100% after being exposed to 450 and 225 mg/ml of A. adscendens extract for 20 and 30 min and in ex vivo for 30 and 60 min, respectively. Following 48 h treatment of protoscoleces, A. adscendens chloroformic extract at the doses of 56.25, 112.5, 225, and 450 mg/ml, dose-dependently motivated the caspase-3 enzyme ranging from 8.8% to 29.6% .
  • Conclusion: A. adscendens root chloroformic extract had a significant protoscolicidal effect; however, extra surveys are required to assess its efficacy and safety as a promising protoscolicidal agent in clinical settings.
  • Keywords: Hydatid cyst, Echinococcus granulosus, Scolicidal activity, Caspase, Herbal medicine.