E-Book 2nd Congress

  • Determination of spatial epitopes on human immunoglobulin light chain by computational immunology
  • Amir Shourideh,1,* Elnaz Sadi,2
    1. Eastern Mediterranean University
    2. Eastern Mediterranean University


  • Introduction: Immunoglobulins are a set of proteins which have a critical function in defense. Against microorganisms. Immunoglobulins include heavy and light chains. In human, Immunoglobulin light chain comprises of isotypes: Kappa (k) and lambda (λ) based totally on amino acid differences in carboxylic end in their consistent area. Marked modifications in the k to λ ratio can occur in monoclonal expansion of neoblastic B cells or HIV infection in neonatal periods. Highly sensitive and particular anti-light chain MAbs have clinical importance inside the diagnosis and immunotherapy of patients with B-cell immunoproliferative diseases. Therefore, specific dedication of unique epitopes in mild chains could be very important. Computational immunology makes use of the computational data for more accurate diagnosis of diseases. This study describes determination of conformational epitopes in constant area of human immunoglobulin light chain via computational immunology.
  • Methods: The amino acid residue and third structure of reference human immunoglobulin G light chain become observed in PDB database. The second immunoglobulin G structure become described by Phyre 2 software. Conformational epitopes of the immunoglobulin light chain have been specified with the aid of using CEP software.
  • Results: In this study, the CEP software identified five conformational epitopes located in the constant domain of the human immunoglobulin light chain. These conformational epitopes were located in light chain sequences of 100-214 amino acids.
  • Conclusion: In this study, several conformational epitopes located in the constant domain of the human immunoglobulin light chain were identified. These epitopes are valuable tools for generating specific anti-immunoglobulin light chain monoclonal antibodies and could have a potential impact on the production of specific human immunoglobulin light chain diagnostic kits, the control of monoclonal light chain diseases and the treatment of related B-cell tumors have, immunoglobulin light chain epitope mapping and evolution studies.
  • Keywords: Human Immunoglobulins, Light chains, Conformational epitope, Computational immunology