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  • The effect of green synthesized zinc oxide nanoparticles on the cell death of acute lymphoblastic leukemia cell line
  • Muhammad Hossein Ashoub,1 Fahime Mahmoudi,2 Mahnaz Amiri,3,*
    1. Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran
    2. Faculty of agriculture, Horticultural science, and engineering, medicinal plants, Shahid Bahonar University of Kerman, Iran
    3. Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran


  • Introduction: Leukemia therapy via nanomedicine strategies, specifically via nanoparticle-based compounds used to deliver drugs, diagnose, and induce cell death, are prospective methods in the near future. Owing to the exceptional properties of green alkalinized agents, such as minor toxicity, higher biodegradability, high active surface, and environmental compatibility, in the present work, the green alkalinized agent (Satureja mutica) used to prepare zinc oxide nanoparticles (ZnNPs). In addition, the toxicity of synthesized NPs on the cell death of acute lymphoblastic leukemia cell line was also evaluated.
  • Methods: ZnNPs synthesized by the precipitation method in the presence of Satureja mutica plant extract as a green precursor acted as a reducing and capping agent. XRD, TEM, SEM, FT-IR, and DLS techniques used to characterize NPs. The Nalm-6 cell line (ALL) and PBMCs (Normal cells) treated with different concentrations of zinc oxide NPs for 24, 48, and 72 hours. Afterward, the cell viability and metabolic activity evaluated via trypan blue and MTT assays. In addition, Gene's expression was assessed using qRT-PCR.
  • Results: Our findings revealed that ZnNPs applied growth inhibitory effects in a dose- and time-dependent manner. In comparison, they had no significant impact on the viability of PBMCs. Moreover, Gene's expression analysis for apoptotic genes displayed a remarkable elevation in the Bax and a reduction in Bcl-2 genes compared to the control group.
  • Conclusion: Given the apoptotic effects of the zinc oxide NPs on leukemic cells, our study suggests a promising therapeutic strategy for ALL treatments.
  • Keywords: ALL; Zinc oxide; Hydrothermal; Herbal extracts; Apoptosis.