E-Book

E-Book 2nd Congress

  • Histopathological effects of different doses of Hexaflumuron on the liver of Wistar rats
  • Nadia Naseri,1 Seyed Mohammad Hosseini,2,*
    1. Department of pathology,Islamic Azad University,Babol branch,Babol,Iran
    2. Department of pathology,Islamic Azad University,Babol branch,Babol,Iran


  • Introduction: Pesticide use has recently increased due to population expansion and increased food demand. Hexaflumuron is a systemic pesticide of the benzoyl urea group that kills insects by preventing the formation of chitin in their bodies. This insecticide is used to control a wide range of plant pests and is more effective on the immature stages of insects. The liver is part of the body's reticuloendothelial system and It can effectively eliminate the toxic effect of agents entering the body.Only a few data are available related to the effects of Hexaflumuron on animals; this study investigated Histopathological effects of different doses of Hexaflumuron on the liver of Wistar rats
  • Methods: sixteen rats were divided in four groups. Group A presented as standard control. Group B, C and D were administered hexaflumuron by gavage with different doses (22, 16.5, 11 mg/kg corresponding to 20% of LD50, 15% of LD50, 10% of LD50) for 28 days. Immediately after sampling, the liver tissues were washed with sterile normal saline, placed in 10% formalin buffer and then processed for routine paraffin embedding. Stained by H&E and examined under a light Olympus microscope to determine any pathological alterations.
  • Results: Liver tissue sections of the control rat showed normal histological structure. The high dose group (20% of LD50) demonstrated the most congestion and infiltration with inflammatory cells. The 10%of LD50 group showed the lowest amount of congestion, we didn't observe infiltration with inflammatory cells in this group.
  • Conclusion: These findings show that the liver of the Hexaflumuron receiving group showed moderate to severe histopathological alterations, so special attention should be addressed to the harmful effects of this insecticide on the liver.
  • Keywords: Hexaflumuron, histopathology, liver, Wistar Rats