E-Book 2nd Congress

  • Evaluation of the difference in melting temperature of different strains of corona virus
  • Hooshang Hosseinpour Hasan Kiadeh,1 Ali Asghar Majrouhi Sardroud,2,* Majid Mesghar Tehrani,3
    1. Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e- Rey Branch, Islamic Azad University, Tehran, Iran
    2. Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e- Rey Branch, Islamic Azad University, Tehran, Iran
    3. Biotechnology Research Center, Shahid Beheshti University of Medical Scienses, Tehran, Iran


  • Introduction: The Covid-19 disease, which is caused by a viral pathogen called SARS-Cov-2, has caused one of the biggest recent pandemics in the world. Considering the creation of different strains of the SARS-Cov-2 virus from beta, gamma, delta and omicron in the world and the rapid spread of the corona virus, creating a fast and accurate diagnosis method with the aim of controlling possible sources of infection, in order to design effective measures for Preventing further transmission is considered vital. It is also important to know the physiological structure of each virus strain to deal with it. One of the most important factors in investigating the physiological structure of the virus is the detection of the melting point of each strain, which was investigated using the technique of determining the biological melting point.
  • Methods: In this research, beta, gamma, delta and omicron strains, which were prepared from Pasteur Institute of Iran, were used. To measure the biological melting point, a fully intelligent and automatic melting point device of Nasim tashkhis azma Company was used. Spectrophotometry technique was used to validate the test process and check the complete removal of the virus from the viral cell culture process and also to check the removal of the virus genome (RNA) structure.
  • Results: According to this research, the alpha strain of the coronavirus at a temperature of 94.444 degrees Celsius, the beta strain of the coronavirus at a temperature of 78.222 degrees Celsius, the gamma strain of the coronavirus at a temperature of 75.222 degrees Celsius, the delta strain of the coronavirus at a temperature of 96.666 degrees Celsius and the strain Omicrons of the coronavirus reached the melting point and complete removal of cells at a temperature of 76.666 degrees Celsius.
  • Conclusion: Considering the aggressive behavior of alpha and delta strains compared to the other three strains and on the other hand the high ratio of the melting point of these two strains to the other three strains, there is a significant relationship between the melting point of the virus and its aggressiveness.
  • Keywords: melting temperature / corona virus strain / evaluation