E-Book 2nd Congress

  • Molecular Characterization of toxication agent Staphylococcus epidermidis strains in the clinical sample in Isfahan
  • Parisa behshood,1,* Fateme Talebi varnosfaderani,2 Mohamad amin niknezhad,3 Samane ansarinia,4 Elahe Tajbakhsh,5
    1. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    2. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    3. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    4. MSC, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    5. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran


  • Introduction: Coagulase-negative Staphylococci and especially Staphylococcus epidermidis are posed as an emerging cause for the occurrence of hospital infections. The present investigation was done to study the pattern of antibiotic resistance and the prevalence of virulence genes associated with toxication agent outbreaks in Isfahan
  • Methods: During six- months, 60 clinical specimens to isolated from strains of Staphylococcus epidermidis were screened. Following identification strains, MRSE isolates were isolated by PCR method and, then the antibiotic resistance pattern of them was determined by Kirby – Bauer method. The presence of the sea, seb, sed and, sei genes was analyzed by PCR
  • Results: 45 isolates of Staphylococcus epidermidis were isolated from 60 samples, 30 isolates (66.6 percent) were MRSE. MRSE isolates exhibited the highest rates of resistance to penicillin (80 percent), and cefoxitin (56.6 percent), while they showed the lowest resistance to levofloxacin (13.3 percent), and rifampicin (6.6 percent). The prevalence rate of Moreover, the frequency of enterotoxin genes sea, seb, sed and, sei was 60 percent, 63.3 percent, 13.3 percent and, 76.6 percent respectively, in the isolate.
  • Conclusion: In this study, the level of gene expression and antibiotic resistance was high. Therefore, it is necessary to investigate these strains in order to control infection.
  • Keywords: toxication agent-Staphylococcus epidermidis-clinical sample